Recombinant Human ALK-2/ACVR1 Protein (His & Fc Tag)(Active)

Size: 200μg

Catalogue Number: PKSH031744-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: ALK-2; ACVR1

Target Symptom: Activin Receptor Type-1; Activin Receptor Type I; ACTR-I; Activin Receptor-Like Kinase 2; ALK-2; Serine/Threonine-Protein Kinase Receptor R1; SKR1; TGF-B Superfamily Receptor Type I; TSR-I; ACVR1; ACVRLK2; ACVR1A; ACVRLK2; ALK2; FOP; SKR1

Research Area: Signal Transduction; epigenetics and nuclear signal; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His & Fc

Accession: NP_001104537.1

Background: ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).

Activity: Measure by its ability to bind with human BMP2 in a functional ELISA.

Sequence: Met 1-Val 124

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 39.6 kDa

Observed MW: 40-45 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only