DC Spyfall is an easy-to-learn party game based on the social party game Spyfall. It contains elements of bluff, questions and clever answers. At the start of each round, players receive a secret card containing information about the group's location - one of twenty unique DC locations, including Arkham Asylum, the Daily Planet, the Hall of Justice and STAR Labs - except for one player, who receives the Joker card instead. The Joker doesn't know where he is, but if he can guess his location before his cover is discovered, he wins the round!

DC Spyfall adds some twists to the Spyfall format. Since Harley Quinn usually follows the Joker, one player can receive a location card with a Harley image in each round. If this is the case, they will try to give the Joker player information about the location, even if they don't know that player's identity! If the Joker player guesses the location, the two collaborators score points - unless the superheroes can turn the tables and determine who Harley is.

The game features two multiverse decks with eight different locations each instead of just one, which can confuse players with some questions and answers. Keep in mind that no one at the table knows if they are playing with a multiverse deck. A full Joker deck gives each player a Joker card, meaning all questions and answers are based on nothing more than what the players collectively believe. Just like with the multiverse decks, you don't know you're playing this deck until someone discovers it.

Super power cards can give a player an ability for a round, such as dodging a question, or require a player to adjust their behavior; for example, Super Speed ​​forces a player to answer a question in three words or less.