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J MediumJ Medium Modification of Parker's J (or, Jansen) medium for Microcystis. Directions For 1 L Total pH 8. 3 8. 5 To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. For 1. 25% agar medium add 12. 5 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final
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J Medium

Modification of Parker's J (or, Jansen) medium for Microcystis.

Directions

For 1 L Total pH 8.3 - 8.5
  1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
    • For 1.25% agar medium add 12.5 g of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Macro Component 1 for J Medium 10 mL/L
2 Macro Component 2 for J medium 10 mL/L
3 CaCl22H2O
(Sigma C-3881)
1 mL/L 0.29 g/100 mL dH20 0.02 mM
4 G9 Trace Metals for J medium 1 mL/L

J Medium

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