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Volvox MediumVolvox Medium Modification of the original recipe. Suitable for most axenic strains of Volvox and some of Eudorina and Pandorina. Not suitable for xenic cultures. For additional modifications of Volvox Medium, see HEPES volvox, MES volvox, P49, and Volvox Dextrose. Directions For 1 L Total To approximately 950 mL of dH2O, add the following components in the order listed (except vitamins) while stirring continuously. Adjust the pH to 8. 0 Bring total

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Volvox Medium

Modification of the original recipe. Suitable for most axenic strains of Volvox and some of Eudorina and Pandorina. Not suitable for xenic cultures. For additional modifications of Volvox Medium, see HEPES-volvox, MES-volvox, P49, and Volvox-Dextrose.

Directions

For 1 L Total

To approximately 950 mL of dH₂O, add the following components in the order listed (except vitamins) while stirring continuously.
Adjust the pH to 8.0
Bring total volume to 1 L with dH₂O.
- For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
Cover and autoclave medium.
Allow to cool and add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Ca(NO₃)₂•4H₂O (CAS: 13477-34-4)	1 mL/L	11.8 g/100 mL dH₂O	0.5 mM
2	MgSO₄•7H₂O (CAS: 10034-99-8)	1 mL/L	4 g/100 mL dH₂O	0.16 mM
3	Na₂glycerophosphate•5H₂O (CAS: 13408-09-8)	1 mL/L	5 g/100 mL dH₂O	0.16 mM
4	KCl (CAS: 7447-40-7)	1 mL/L	5 g/100 mL dH₂O	0.67 mM
5	Glycylglycine (CAS: 556-50-3)	10 mL/L	5 g/100 mL dH₂O	3.8 mM
6	P-IV Metal Solution	6 mL/L
7	Vitamin B₁₂	1 mL/L
8	Biotin Vitamin Solution	1 mL/L