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Allen MediumAllen Medium Modification of M. M. Allen's freshwater medium for blue greens. Suitable for axenic and xenic cultures. The marine LB 1928 Spirulina platensis [Medium: Enriched Seawater (ES)] can be adapted to Allen medium (D. James 1982, pers. Comm.). Directions For 1 L Total To approximately 950 mL of dH2O add each of the components in the order specified while stirring continuously. Adjust pH to 7. 8. Bring total volume to 1 L with dH2O. For 1% agar
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Allen Medium
Modification of M.M. Allen's freshwater medium for blue-greens. Suitable for axenic and xenic cultures. The marine LB 1928 Spirulina platensis [Medium: Enriched Seawater (ES)] can be adapted to Allen medium (D. James 1982, pers. Comm.).
Directions
For 1 L Total
- To approximately 950 mL of dH2O add each of the components in the order specified while stirring continuously.
- Adjust pH to 7.8.
- Bring total volume to 1 L with dH2O.
- For 1% agar medium: add 10 g of agar to the flask; do not mix.
- Cover and autoclave medium.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | HEPES buffer (Sigma H-3375) |
2.3 g/L | 0.01 M | |
| 2 | NaNO3 (Fisher BP360-500) |
1.5 g/L | 0.018 M | |
| 3 | P-IV Metal Solution | 1 mL/L | ||
| 4 | K2HPO4 (Sigma P 3786) |
5 mL/L | 1.5 g/200 mL dH2O | 0.215 mM |
| 5 | MgSO4•7H2O (Sigma 230391) |
5 mL/L | 1.5 g/200 mL dH2O | 0.152 mM |
| 6 | Na2CO3 (Baker 3604) |
5 mL/L | 0.8 g/200 mL dH2O | 0.19 mM |
| 7 | CaCl2•2H2O (Sigma C-3881) |
10 mL/L | 0.5 g/200 mL dH2O | 0.17 mM |
| 8 | Na2SiO3•9H2O (Sigma 307815) |
10 mL/L | 1.16 g/200 mL dH2O | 0.204 mM |
| 9 | Citric Acid•H2O (Fisher A 104) |
1 mL/L | 1.2 g/200 mL dH2O | 0.029 mM |
Allen Medium
Item no
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42975754716
US$ 55.00
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