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Modified Artificial Seawater MediumModified Artificial Seawater Medium Modification of artificial seawater medium for B 2795 Picochlorum oklahomensis. Directions For 1 L Total To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously. Adjust the pH to 8. 0. Bring the total volume to 1 L with dH2O. For 1. 5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add vitamins.

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Modified Artificial Seawater Medium

Modification of artificial seawater medium for B 2795 Picochlorum oklahomensis.

Directions

For 1 L Total

To approximately 850 mL of dH₂O, add each of the components in the order specified (except vitamins) while stirring continuously.
Adjust the pH to 8.0.
Bring the total volume to 1 L with dH₂O.
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
Cover and autoclave medium.
When cooled add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	NaCl (Fisher S271-500)	30 g/L		0.51 M
2	MgSO₄•7H₂O (Sigma 230391)	10 mL/L	24.4 g/100 mL dH20	9.9 mM
3	KCl (Fisher P 217)	10 mL/L	6 g/100 mL dH20	8 mM
4	NaNO₃ (Fisher BP360-500)	10 mL/L	10 g/100 mL dH20	11.8 mM
5	CaCl₂•2H₂O (Sigma C-3881)	10 mL/L	3 g/100 mL dH20	2.04 mM
6	KH₂PO₄ (Sigma P 0662)	10 mL/L	0.5 g/100 mL dH20	0.37 mM
7	Tricine (Sigma T-5816-256)	10 mL/L	10 g/100 mL dH20	5.58 mM
8	NH₄Cl (Fisher A 649-500)	1 mL/L	2.7 g/100 mL dH20	0.5 mM
9	P-IV Metal Solution	1 mL/L
10	Soilwater: GR+ Medium	30 mL/L
11	Vitamin B₁₂	1 mL/L
12	Biotin Vitamin Solution	1 mL/L
13	Thiamine Vitamin Solution	1 mL/L

Modified Artificial Seawater Medium

Name: Modified Artificial Seawater Medium
Brand: oderzo.it
SKU: 32849216635
Price: 55.00 USD
Availability: InStock
Rating: 4.488700000000001 (2471 reviews)